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DNA fluorescence flow cytometric profiles of alcohol-fixed, PI-stained human colonic lamina propria cells cultured in control medium or in the presence of C. difficile toxin A. Apoptotic nuclei produce broad hypodiploid peaks, which can be easily distinguished from the tall narrow peaks produced by cells with normal DNA content. For control versus toxin A-exposed cells, P = 0.001. The figure shown presents results representative of three experiments.
