FIGURE 4.

The NTD is essential for MR tetramerization in the nucleoplasm. (a) Schematic representation of MR deletion and domain swapping constructs. NTD, N‐terminal domain; DBD, DNA binding domain; LBD, ligand binding domain. (b) Representative images of single cell nuclei expressing the indicated constructs. White arrows point to the MMTV array. Scale bars: 5 μm. (c) N&B results obtained with the indicated constructs. To facilitate comparison, data from Figure 1 showing ε for GR‐N525 and MR treated with 10 nM aldosterone are included. Data points correspond to ε obtained from a single nucleus (n = 490, 307, 82, 47, 42, 22, 43, 22, 26, 11, 35 and 23 cells in each condition, from left to right). Individual points were pooled from the following number of independent experiments for each condition: 25, 5, 2, 2, 2, 2. Horizontal bars represent mean ±95% CI. Each ε value was compared to its reference value (wild type MR in the nucleoplasm or at the MMTV array) using the Kruskal‐Wallis test followed by Dunn's multiple comparisons correction. Symbols refer to statistical differences with MR wild type in the same compartment (nucleoplasm or MMTV array; n.s., non‐significant; **p < 0.01).