FIGURE 5.

Polyclonal phage ELISA of the outputs from each phage display round (a–c). (a) Cross‐panning with recombinant toxins (rLr 100 nM, rHl 100 nM, rHl 50 nM). (b) Cross‐panning with recombinant toxins (rHl 100 nM, rLr 100 nM, rLr 50 nM). (c) Stringent panning with cSMase (100 nM, 50 nM, 10 nM). The bars represent an average of triplicates, and the error bars represent the standard deviation of the measurement. In each round, five different antigens were assayed: the three recombinant proteins rLr (black), rHl (pink), and the cSMase (green), and two negative controls: the native unrelated toxin α‐cobratoxin (α‐cbtx) (purple), purified from the venom of Naja kaouthia, and streptavidin (Strp) (lilac).