Figure 7.

The detection of anti-biotin IgG using the biotinylated polyolefin nonwoven. (a) Scheme for the IgG detection. Absorbance at 490 nm of the HRP-catalyzed reaction products from (b) HRP-conjugated anti-biotin IgG and (c) HRP-conjugated anti-mouse IgG IgG adsorbed on the biotinylated nonwoven and control samples. IgGs were applied at 100 ng mL⁻¹ to nonwovens. The leftmost bars in (b) and (c) (azidated cello-oligosaccharides with biotin (+) and linker (+)) represent the results for the biotinylated nonwovens, and ‘linker’ denotes BCN-amine. The absorbance values are presented as the average of nine individual trials, and the error bars represent the standard deviation of those trials. (d) Effect of IgG concentration on absorbance at 490 nm for detection. The absorbance values are presented as the average of three individual trials, and the error bars represent the standard deviation of those trials.