Figure 2.

HPP induced bladder cancer cell autophagy in the co-culture system. A-B Protein expression of p62, Beclin1, and LC3II/I was determined by Western blotting analysis. C-F MB49 cells were pre-treated with 5 mM 3-MA for 4 h or 50 μM CQ for 3 h, and then co-cultured with RAW264.7 cells in fresh medium containing 100 μg/mL HPP. Protein expression of LC3II/I was determined by Western blotting analysis. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control group. ^##p < 0.01 compared with the HPP group.