Figure 3.

hTRPV4 interactions with RhoA GTPase. a, cartoon representation of the RhoA structure. The RhoA protein is bound to GDP (green sticks). b, the homotetramer of the human TRPV4 in complex with RhoA is shown; each subunit of TRPV4 is identified with a different color, RhoA (wheat) is shown interacting with the TRPV4 bottom layer; the stoichiometry is 1:1. c, lateral view of two subunits of TRPV4 in complex with RhoA and a close-up view of the interaction zone between the ARD of hTRPV4 (yellow sticks) and the β sheets of RhoA (blue sticks). PDB: 8FC9 (resolution 3.75 Å) [52,53]. Created with PyMOL and BioRender.com.