Figure 5.

Pore domain of TRPV4. the pore regions of the TRPV4 channel from a, frog, b, mouse, and c, human are shown in the apo state; all of them have a selectivity filter in the upper region which differs in their cross-pore distances. The lower region of the pore domain contains the intracellular gate, where the distance between the side chains of the nearest amino acids is less than 6 Å, preventing the passage of ions. Mammalian ion TRPV4 channels show similarity in structure in contrast to the frog TRPV4, which displays a “tighter” conformation. The amino acids in the selectivity filter and the intracellular gate are shown as sticks. PDB: 6BBJ, 8J1D, and 8T1B (resolutions of 3.80Å, 3.59 Å and 3.00 Å, respectively) [45,52,127]. Created with PyMOL and BioRender.com.