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. Author manuscript; available in PMC: 2024 Feb 15.
Published in final edited form as: Nat Cell Biol. 2023 Oct 16;25(11):1691–1703. doi: 10.1038/s41556-023-01253-2

Extended Data Fig. 4 |. Cell biological and transcriptional effects of Ifh1 depletion during heat shock.

Extended Data Fig. 4 |

(a) Immunoblot showing of the level of Ifh1-mAID-3xFlag upon incubation with 5ph-IAA and β-estradiol. PGK1 level is used as loading control between the samples. (b) HSE-YFP reporter heat shock time course showing reduced HSR induction when Ifh1 is depleted. Data are presented as mean ± S.D. n = 3 biologically independent sample. (c) RT-qPCR of the HSR target gene transcript SSA4 over a heat shock time course in the absence and presence of Ifh1 depletion. Data are presented as mean ± S.D n = 3 biologically independent sample. (d) LLS live-imaging of yeast cells with endogenously tagged Sis1-mVenus (green), Hsp104-TFP (blue), Sec61-Halo (red) and Nsr1-mScarlet-I (white) under non-stress (30 °C) and heat shock (39 °C, 10 min) in the absence and presence of Ifh1 depletion. (e) Quantification of Sis1 cytosolic foci per cell in the conditions shown in (d). Statistical significance was assessed using the Brown-Forsythe and Welch ANOVA test, along with Games-Howell multiple post hoc comparisons. n denotes number of cells from 3 independent experiment.