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. 2024 Feb 5;20(2):e1010527. doi: 10.1371/journal.pgen.1010527

Fig 2. SSA repair frequency.

Fig 2

A. Both mismatches and nonhomologous tails reduce SSA. The frequency of DSB-induced SSA was measured after induction of GAL::HO (in Tailed strains) or GAL::CAS9 + gRNA (in Tailless strains) compared to cells grown on dextrose. Statistical test: One-Way ANOVA; Error bars represent the standard deviations; **** = p< 0.0001. B. Constitutively-expressed Cas9 was compared with GAL::HO induction of SSA in a tailed strain. Cas9 targets a site near the HO DSB (DSB-1 in panel C). Experiments were independently repeated at least five times. Error bars indicate standard deviations. Experimental means were statistically compared by the unpaired t-Test. C. Comparison of GAL::HO and GAL::CAS9 induced SSA when Cas9 cleaves the same sequence used in the Tailless construct (DSB-2 in red). D. Strains with only one nonhomologous tail were compared with 2-tail and Tailless strains using GAL::CAS9.