Fig 10. Plasmid transformation assay in rad52Δ and wild type strains.

A plasmid containing 200 bp repeats, URA3, and CEN6 was transformed into wild type and rad52Δ strains. The plasmid was cut with the restriction enzymes shown to mimic one-tail, two-tail or tailless SSA. Survival frequencies are shown for the transformation normalized to the uncut plasmid and to the highest transformation frequency, obtained when the DNA was cut with HindIII + SphI.