Table 1. Cell viability data collected from Gal inducible assay.
The effect of MMR proteins, helicases and recombinases on SSA repair efficiency. GAL::HO and GAL::Cas9 were used in the Tailed and Tailless strains, respectively. The frequency of survival after an HO or Cas9 induced DSB was calculated by dividing the number of colonies growing on YEP–GAL by the number of colonies growing on YEPD. Each value represents the average from at least five independent experiments. One SD of the mean is shown in parentheses. ND = not determined.
| % Viability | ||||
|---|---|---|---|---|
| Divergent repeats | Identical repeats | |||
| Genotype | FA Tailed | FA Tailless | AA Tailed | AA Tailless |
| WT | 26 (3.1) | 85 (9.4) | 68 (5.0) | 100 (6.3) |
| sgs1△ | 68 (12.0) | 98 (3.8) | 100 (3.2) | 100 (8.2) |
| msh6△ | 59 (7.6) | 92 (3.2) | ND | ND |
| msh2△ | 10 (1.9) | 53 (8.4) | 3 (1.6) | ND |
| msh3△ | 2.3 (0.8) | 88 (16.7) | 3 (1.9) | 97% (10) |
| mlh1△ | 28 (3.3) | 81 (8.4) | 69 (9.3) | 91 (8.4) |
| pms1△ | 22 (2.8) | 73 (9.3) | 57 (11.0) | 98 (5.2) |
| exo1△ | 31 (3.6) | 50 (6.1) | ND | ND |
| srs2△ | 0.8 (0.0) | ND | ND | ND |
| rad1△ | 1.5 (0.1) | 61 (5.8) | 1.3 (0.1) | 77 (6.8) |
| rad△ | 42 (5.7) | N.D | 72 (7.6) | N.D |
| rad52△ | 0.2 (0.0) | N.D | 0.2 (0.10) | N.D |