FIG. 1.

iNOS mRNA expression in spleens of H. capsulatum-infected mice coincides with active infection. Total RNA extracted from normal (lane 1) and infected mice on days 10 (lane 2), 35 (lane 3), and 55 (lane 4) after infection. RNA was reverse transcribed and cDNA was amplified by PCR with paired iNOS primers and HPRT primers simultaneously. The arrowhead points to HPRT PCR products (352 bp), and the arrow points to iNOS PCR products (306 bp). The ratios of iNOS to HPRT are 0.12, 0.77, 0.39, and 0.21 for lanes 1, 2, 3, and 4, respectively.