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Splenic macrophages express iNOS protein in H. capsulatum-infected mice. Spleen cryosections from H. capsulatum-infected (2 to 3 weeks postinfection) (A) and normal (B) mice were stained with FITC-conjugated polyclonal rabbit anti-mouse iNOS antibody. (A) The arrow points to a cell, most probably a macrophage or giant cell by morphology, which stains positive for iNOS. (B) The spleen section was stained with ethidium bromide as a counterstain. Magnification, ×400.
