TABLE 1.
Source of splenocytesa | Stimulant | Level of nitrite (μM)d |
---|---|---|
Normal mice | None | 2.3 |
ConAb | 0.5 | |
H. capsulatumc | 1.1 | |
Infected mice | None | 13.6 |
ConA | 21.1 | |
H. capsulatum | 18.4 |
Splenocytes from normal and H. capsulatum-infected mice at 2 to 3 weeks after infection were cultured at 107 cells per ml. Supernatant fluids were collected 24 h after incubation.
ConA was added at 2 μg/ml.
Heat-killed H. capsulatum yeasts were added at a 1:40 splenocyte-to-yeast ratio.
These data are representative of three separate experiments.