TABLE 2.
Effect of NO on the proliferative response of splenocytes from H. capsulatum-infected mice
| Source of splenocytea | NMMAb | ConAb | Heat-killed H. capsulatumc | SId |
|---|---|---|---|---|
| Normal mice | − | − | − | 1.0 |
| − | + | − | 7.3 | |
| − | − | + | 1.2 | |
| + | − | − | <1.0 | |
| + | + | − | 7.3 | |
| + | − | + | 1.3 | |
| Infected mice | − | − | − | 1.0 |
| − | + | − | <1.0 | |
| − | − | + | 1.7 | |
| + | − | − | 7.3 | |
| + | + | − | 21.5 | |
| + | − | + | 9.0 |
a
Splenocytes from normal and H. capsulatum-infected mice at 2 to 3 weeks after infection were cultured at 5 × 105 cells in a 96-well plate.
b
NMMA was added at 1.2 mM. Con A was added at 2 μg/ml.
c
Heat-killed H. capsulatum yeasts were added at a 1:40 splenocyte-to-yeast ratio.
d
The stimulation index (SI) is the counts per minute (cpm) of splenocytes from either normal or infected mice stimulated with ConA or heat-killed yeast cells divided by the cpm of splenocytes from either normal or infected mice without stimulation. Background thymidine uptakes were 2,934 ± 580 for normal splenocytes and 2,250 ± 982 for splenocytes from infected mice.