Table 2.
Primers for RT-qPCR analysis of tissue RNA.
| Gene names | Primers sequence 5′→3′ | Accession no. |
|---|---|---|
| Claudin-1 | F: AAGTGCATGGAGGATGACCA R: GCCACTCTGTTGCCATACCA |
NM_001013611.2 |
| Occludin | F: AGTTCGACACCGACCTGAAG R: TCCTGGTATTGAGGGCTGTC |
NM_205128.1 |
| ZO-1 | F: ACAGCTCATCACAGCCTCCT R: TGAAGGGCTTACAGGAATGG |
XM_015278981.1 |
| IL-1β | F: TCATCTTCTACCGCCTGGAC R: GTAGGTGGCGATGTTGACCT |
NM_204524.1 |
| IL-2 | F: GAGTGCACCCAGCAAACTCT R: CCGGTGTGATTTAGACCCGT |
NM_204153.1 |
| IL-4 | F: GTGCCCACGCTGTGCTTAC R: AGGAAACCTCTCCCTGGATGTC |
NM_001007079.1 |
| IL-8 | F: GGCTTGCTAGGGGAAATGA R:AGCTGACTCTGACTAGGAAACTGT |
NM_205498.1 |
| TNF-α | F: CCCCTACCCTGTCCCACAA R: TGAGTACTGCGGAGGGTTCAT |
NM_204267.1 |
| TGF-β2 | F: TCATCACCAGGACAGCGTTA R: TGTGATGGAGCCATTCATGT |
NM_001031045.3 |
| NF-κB | F: TGGAGAAGGCTATGCAGCTT R: CATCCTGGACAGCAGTGAGA |
NM_205134.1 |
| MyD88 | F: TGCAAGACCATGAAGAACGA R: TCACGGCAGCAAGAGAGATT |
NM_001030962.3 |
| TLR4 | F:GATGCATCCCCAGTCCGTG R:CCAGGGTGGTGTTTGGGATT |
NM_001030693 |
| β-actin | F: GAGAAATTGTGCGTGACATCA R: CCTGAACCTCTCATTGCCA |
NM_205518.1 |
Abbreviations: IFN-γ, interferon-γ; IL-1β, interleukin-1β; IL-2, interleukin-2; IL-4, interleukin-4; IL-8, interleukin-8; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor κB; TGF-β2, transforming growth factor-β2; TLR4, Toll-like receptor 4; TNF-α, tumor necrosis factor-α; ZO-1, zonula occludens-1.