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. 2024 Feb 15;15(2):145. doi: 10.1038/s41419-024-06503-1

Fig. 2. Cystine deprivation triggers evident ferroptosis and exhaustion of CD8+ T cells.

Fig. 2

a, b T cells were cultured in 10 μmol/L cystine medium containing Fer-1, NAC, Z-VAD, NEC-1, and MCC950, respectively (n = 3 per group). Cell viability was detected via CCK8 assay (a), and lipid peroxidation was measured via C11 BODIPY staining (b). c, d RNA-seq analysis of ferroptosis activation-related genes, memory/effector-related genes and dysfunction/exhaustion-related genes of T cells cultured in NM or CD (n = 3 per group). e, f Flow cytometry analysis of CD62L expression, PD-1+TIM-3+ T-cell subset, cytokine secretion (e) and TCF1 expression (f) of the indicated T cells (n = 3 per group). Each symbol represents one individual. Data are presented as mean ± s.e.m. p values are measured by one-way ANOVA with Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001.