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. 2024 Feb 15;15(2):145. doi: 10.1038/s41419-024-06503-1

Fig. 3. Inhibition of cystine uptake by tumor cells alleviates ferroptosis of CD8+ T cells and enhances anti-tumor immunity.

Fig. 3

a Western blot analysis of Slc7a11 expression in WT and Slc7a11-KD B16F10 cells. b CCK8 assay measuring cell viability of WT and KD B16F10 cells after 2 h and 48 h in vitro culturing (n = 4 per group). c, d Tumor volume (c) and tumor weight (d) of WT and KD B16F10 tumors (n = 6 per group). e Flow cytometry measuring the percentages of tumor-infiltrating CD8+ T cells in WT and KD tumors. f The percentages of dead CD8+ T cells in WT and KD tumors. g Lipid peroxidation of CD8+ T cells in WT and KD tumors. h PD-1 expression of CD8+ T cells in WT and KD tumors (one outlier was removed from KD). i Levels of IFNγ, TNFα, and IL-2 secretion of the indicated T cells. j Tumor volume of WT and KD tumors after CD8 antibody administration (WT: n = 4; KO: n = 5; WT + αCD8: n = 5; KD + αCD8: n = 6). Each symbol represents one individual. Data are mean ± s.e.m. p values are measured by two-tailed unpaired Student’s t test. Ns not significant, *p < 0.05, **p < 0.01.