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. 2024 Feb 15;10:79. doi: 10.1038/s41420-024-01845-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A 2D in vivo fluorescent images of NMRI Nudes mice and B ex vivo fluorescent signal of organs after intravenous injection of AlexaFluor 680 labeled Trastuzumab-Qtag2-MMAE (Mean ± SEM (n = 4)). Immunofluorescent analysis of BT-474 tumor sections after intraperitoneal injection of C vehicle control or Trastuzumab-Qtag2-DM1 D on day 1 and E day 3. The green signal represents the homing of ADC using AlexaFluor488 labeled anti-DM1 mouse monoclonal antibody. Blue, DAPI (nucleus). F Quantitation of the ADCs concentrations in mouse serum (15 mg/kg of ADC, BT-474-Her2 positive tumor) was performed by competition ELISA assay. 96-well plates were coated with anti-DM1 mAb to capture ADC-DM1 and HRP-labeled DM1. The detected absorbance is inversely proportional to the amount of ADC-DM1 conjugate in the test sample (Mean ± SEM (n = 3). Statistical significance was evaluated using GraphPad Prism 7.0b (GraphPad Software Inc., San Diego, CA, USA) software using unpaired one tailed t-test, N.S. non significative.