Table 1.
CT26 neoantigens encoded by Ad-CT26 vaccine
| Position | CHR coord (mm10) | Mapping | NeoAg sequence (reference/mutation) | Best IC50 mutation (nM) | MHC class I |
|---|---|---|---|---|---|
| 1 | 16:57133128 | Tomm70a | AQAAKNKGNKYF[K/Q]AGKYEQAIQCYT | 459.45 | H-2-Kd |
| 2 | 1:33777642 | Zfp451 | VLLYCHISEGSR[P/L]PCDLHLFSQPKI | 883.69 | H-2-Kd |
| 3 | 5:48219745 | Slit2 | QDLHNLNLLSLY[D/A]NKLQTVAKGTFS | 206.17 | H-2-Kd |
| 4 | 1:66706508 | Rpe | GHPVVESLRKQL[G/D]QDPFFDMHMMVS | 503.57 | H-2-Ld |
| 5 | 14:34343682 | Glud1 | LRTAAYVNAIEK[V/I]FKVYNEAGVTFT | 141.15 | H-2-Kd |
| 6 | 2:129208197 | Slc20a1 | KPLRRNNSYTSY[T/I]MAICGMPLDSFR | 118.03 | H-2-Kd |
| 7 | 1:30804436 | Phf3 | PGPQNFPPQNMF[G/E]FPPHLSPPLLPP | 41.5 | H-2-Ld |
| 8 | 12:51365554 | G2e3 | FEGSLAKNLSLN[S/F]QAVKENLYYEVG | 467.97 | H-2-Kd |
| 9 | 7:48871007 | E2f8 | ILPQAPSGPSYA[I/T]YLQPAQAQMLTP | 320.56 | H-2-Kd |
| 10 | 7:55873449 | Cyfip1 | YIEQATVHSSMN[E/K]MLEEGQEYAVML | 929.32 | H-2-Kd |
| 11 | 7:65663891 | Tarsl2 | AIKIFKNSSTYW[E/K]GNPEMETLQRIY | 585.18 | H-2-Kd |
| 12 | 4:86583172 | Haus6 | DSNLQARLTSYE[A/T]LKKSLSKIREES | 18.22 | H-2-Kd |
| 13 | 2:72970297 | Sp3 | ITLTPVQTLTLG[Q/H]VAAGGALTSTPV | 659.55 | H-2-Kd |
| 14 | 1:143699294 | Cdc73 | VWGTGKEGQPRE[Y/H]YTLDSILFLLNN | 799.16 | H-2-Dd |
| 15 | 18:5767633 | Zeb1 | GAQEEPQVEPLD[L/F]SLPKQQGELLER | 121.04 | H-2-Ld |
| 16 | 17:34205757 | Tap2 | LSRPDLPFLIAA[F/V]FFLVVAVWGETL | 86.9 | H-2-Ld |
| 17 | 2:180713221 | Gid8 | MSYAEK[P/S]DEITKDEWMEKL | 67.01 | H-2-Kd |
| 18 | 1:173926892 | Ifi203 | INPTMIISNTLS[E/K]SAIATPKISYLL | 445.82 | H-2-Kd |
| 19 | 10:36993650 | Hdac2 | GAGKGKYYAVNF[P/S]MRDGIDDESYGQ | 39.48 | H-2-Kd |
| 20 | 11:58188928 | Gm12250 | TGLYFRKSYYMQ[N/K]YFLDTVTEDAKV | 103.63 | H-2-Kd |
| 21 | new12:91825363 | Sel1l | LIAHMILGYRYW[A/T]GIGVLQSCESAL | 118.72 | H-2-Kd |
| 22 | 7:35547980 | Nudt19 | IHSPYVYEIYMT[L/F]PSENKHVYPRNY | 852.11 | H-2-Kd |
| 23 | 10:121615052 | Xpot | PRGVDLYLRILM[A/P]IDSELVDRDVVH | 41.56 | H-2-Kd |
| 24 | 7:28814084 | Hnrnpl | VLLFTILNPIYS[I/R]TTDVLYTICNPC | 613.79 | H-2-Kd |
| 25 | 19:38812346 | Noc3l | ALASAILSDPES[H/Y]IKKLKELRSMLM | 101.21 | H-2-Kd |
| 26 | 17:35117867 | Csnk2b | QPMLPIGLSDIP[G/D]EAMVKLYCPKCM | 202.23 | H-2-Ld |
| 27 | 7:45881542 | Kdelr1 | WTFSIYLESVAI[L/M]PQLFMVSKTGEA | 836.76 | H-2-Kd |
| 28 | 17:29332778 | Mtch1 | SWIHCWKYLSVQ[G/S]QLFRGSSLLFRR | 13.46 | H-2-Kd |
| 29 | 4:153960954 | A430005L14Rik | SIFLDDDSNQPM[P/A]VSRFFGNVELMQ | 78.13 | H-2-Ld |
| 30 | 12:59160182 | Ctage5 | ALKKLIYAAKLN[A/T]SLKALEGERNQV | 971.41 | H-2-Kd |
| 31 | 19:53635752 | Smc3 | IETQQRKFKASR[D/A]SILSEMKMLKEK | 250.7 | H-2-Kd |
CHR coord (mm10), chromosome coordinates from mouse genome mm10.
The 31 neoAgs were selected by analyzing the mutanome of CT26. MHC class I binding predictions were performed with IEDB 2.17 software.