FIG. 5.

Western blot of transblotted SDS–7% polyacrylamide gels developed for IgG in serum (1:50) from a rat injected with the GLU peptide construct. Lanes 1 and 2 contain previously electrophoresed S. sobrinus 6715 and S. sobrinus DS guanidine eluates, respectively. Lane 3 contains previously electrophoresed enriched S. sobrinus GBP-2 (0.6 μg). Lane 4 contains molecular standards whose estimated masses (in kilodaltons) are indicated to the right of the blot. The stained S. sobrinus components migrating between the 205- and 116-kDa positions reflect the migration of GTF (see Fig. 1). The stained component migrating to approximately 100 kDa is likely to be degraded GTF.