Figure 4.

Overexpression of CAMK2D variants during prenatal neurodevelopment causes a migration deficit

(A) Representative images of coronal brain slices from P0/P1 pups that were transfected with either CAMK2D^S79N, CAMK2D^P139L, CAMK2D^G210R, CAMK2D^Q274P, CAMK2D^R275H, or CAMK2D^L291F at E14.5, using in utero electroporation. White dashed arrow indicates the SVZ; white arrowheads indicate the CP. DAPI in blue, RFP in red. Scale bar indicates 100 μm.

(B) Cumulative graph indicating the migration pattern from the SVZ to the CP in presence of overexpressed CAMK2D^S79N, CAMK2D^P139L, CAMK2D^G210R, CAMK2D^Q274P, CAMK2D^R275H, or CAMK2D^L291F. Number in parentheses is the number of images used for the quantification. Black dashed line indicates EV (n = 12); gray dashed line indicates CAMK2D^WT (n = 19) overexpression.

(C) Quantification of RFP-positive cells that have successfully migrated to the CP, showing the different effects of the variants on migration. # indicates the levels of significance compared to CAMK2D^WT overexpression. ^∗ indicates the levels of significance compared to the empty vector control (EV). Black dashed line represents EV, gray dashed line represents CAMK2D^WT overexpression. Dots represent data points. Error bars indicate SEM; ^∗∗/##p < 0.01; ^∗∗∗∗/####p < 0.0001.