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. 2023 Dec 22;123(3):277–293. doi: 10.1016/j.bpj.2023.12.018

Table 1.

Summary of experimental conditions used to study CDI

Study Cell typec [Ca2+]ob (mM) pH Agonistb (μM) Buffersa,b (mM) Comments
(46) rat hippocampal neurons (1 day old) 0–50 7.3 10 glycine
0.1–300 NMDA		 10 EGTA or 15 BAPTA,
4 ATP		 Vh = −70 mV
(142) rat hippocampal neurons (1 day old) 0.2 or 2 7.3 10 glycine
10 or 100 NMDA		 5 EGTA or 5 BAPTA or 5 EDTA Vh = −70 mV
(47) HEK293 2 7.4 10 glycine
100 glutamate		 1.1 EGTA,
4 ATP		 Vh = −50 mV,
0.25 mM Ca2+ intracellular
(57) rat hippocampal neurons (neonate) 0.2–2.7 7.2 10 glycine
10 NMDA		 10 EGTA,
2 ATP,
0.3 GTP
(54) rat dorsal horn neurons (1–2 days old) 2 7.3 5 glycine
30 NMDA		 None 0.1 mM Ca2+ intracellular
(72) rat hippocampal neurons (2 days old) 2 7.4 10 glycine
50–300 NMDA		 1.1 BAPTA,
4 ATP,
0.6 GTP		 Vh = −60 mV,
0.1 mM Ca2+ intracellular
rat cerebellar granule neurons (7–8 days old) 2 7.4 10 glycine
50–300 NMDA		 1.1 BAPTA,
4 ATP,
0.6 GTP		 Vh = −50 mV,
0.1 mM Ca2+ intracellular
(52) HEK293 0.2–2 7.25 10 glycine
10–100 NMDA
100–1000 glutamate		 0.1–10 EGTA or 2.4–10 BAPTA,
4 ATP		 FK-506 used in some experiments
(61) HEK293 2 7.4 20 glycine
100 glutamate		 1.1 EGTA,
4 ATP		 0.25 mM Ca2+ intracellular
(71) HEK293 10 7.25 50 glycine
10 NMDA		 0.1 EGTA,
6 ATP
(39) HEK293 1.8 7.3 10 glycine
10 glutamate		 10 EGTA,
4 ATP
rat hippocampal neurons (1–2 days old) 1.8 7.3 10 glycine
20 NMDA		 10 EGTA,
4 ATP
(53) HEK293 10 7.25 100 glycine
100 NMDA		 0.1 EGTA,
6 ATP		 Vh = −50 mV
(56) rat hippocampal neurons (2 days old) 2 7.4 20 glycine 1.1 BAPTA,
4 ATP,
0.6 GTP		 Vh = −60 mV,
0.6 mM Ca2+ intracellular,
monosynaptic cultures
(55) rat hippocampal neurons (12–20 days old) 1.3 7.4 3–10 glycine
300 NMDA		 11 EGTA or 20 BAPTA,
4 ATP		 1 mM Ca2+ intracellular
(90) rat hippocampal neurons (12 days old) 1 7.4 10 glycine
10 NMDA		 10 EGTA Vh = −60 mV,
12 nM free Ca2+ intracellular
(59) HEK293 1 7.25 100 glycine
10–100 NMDA or 1000 glutamate		 0.1 EGTA,
4 ATP		 Vh = −50 mV
(91) rat hippocampal neurons (12 days old) 1 7.4 10 glycine
0.1–10 NMDA		 10 EGTA Vh = −30 to −80 mV
800 nM free Ca2+ intracellular
(92) rat hippocampal neurons (12 days old) 2.5 7.4 10 glycine
0.01–10 NMDA		 10 EGTA Vh = −30 to −80 mV
(143) HEK293 2 7.3 100 glycine
50 NMDA		 10 EGTA,
4 ATP
0.5 GTP		 Vh = −50 mV
(58) HEK293 1.8 7.3 100 glycine
100 glutamate		 10 EGTA,
2 ATP		 Vh = −70 mV
(66) mouse hippocampal neurons (14–21 days old) 2 7.2 none 4 ATP
10 EGTA or 1 BAPTA		 synaptically evoked currents in slice preparations
Mg2+ free or 1.25 mM extracellular
(50) HEK293 0.1–5 8.0 100 glycine
1–1000 glutamate		 0.5–5 EGTA or 5 BAPTA,
4 ATP,
0.3 GTP		 Vh = −80 mV
YFP-CaM overexpression
(63) rat cortical neurons (E16) 1 7.2–7.4 10 glycine
100 NMDA		 10 EGTA or 1 BAPTA and 10 EGTA corrected for LJP,
0.1 mM Ca2+ intracellular
(78) HEK293 2 8.0 100 glycine
1000 glutamate		 5 EGTA,
4 ATP
0.3 GTP		 Vh = −80 mV
YFP-CaM overexpression
rat hippocampal neurons (E18) 2 8.0 100 glycine
1000 glutamate		 5 EGTA,
4 ATP
0.3 GTP		 Vh = −80 mV
(51) HEK293 2 8.0 100 glycine
1000 glutamate		 5 EGTA or 10 BAPTA,
4 ATP,
0.3 GTP		 Vh = −80 mV
YFP-CaM overexpression
(49) HEK293 2 7.4 1000 glycine
1000 glutamate		 01 EGTA,
4 ATP
0.3 GTP		 Vh = −60 mV

LJP, liquid junction potential; Vh, holding potential; [Ca2+]o, extracellular Ca2+ concentration.

a

Intracellular Ca2+/divalent buffers.

b

Dose responses are reported as a range of values.

c

Reported ages are the age of cell harvest (not days in vitro).