Figure 6. Inversion of the Notch3^COIN allele in endothelial cells does not cause osteopenia.

A. DNA was extracted from aorta and tibiae and Notch3^COIN inversion was documented by gel electrophoresis of PCR products obtained with primers specific for the Notch3^INV allele. Arrows indicate the position of the 495 bp amplicon. B. Total RNA was extracted from aorta and tibiae, and expression of the Notch3^PEST and Notch3^ΔPEST mRNA measured by qRT-PCR in 1 month old Cdh5-Cre;Notch3^INV/INV mutants (closed circles) and Notch3^COIN/COIN littermate controls (open circles). Transcript levels are reported as copy number corrected for Rpl38 mRNA levels. Bars represent means and ranges S.D.; n = 3–4 for control and n = 4 Cdh5-Cre;Notch3^INV/INV, all biological replicates. C. Weight in gm and femoral length in mm of 1 month old Cdh5-Cre;Notch3^INV/INV (closed circles) and Notch3^COIN/COIN littermates (open circles). Bars represent means and ranges SD; n = 7 males and n = 10 females for control and n = 14 males and n = 10 females for Cdh5-Cre;Notch3^INV/INV. *Significantly different between control Notch3^COIN/COIN and Cdh5-Cre;Notch3^INV/INV, p < 0.05 by unpaired t-test.