Table 1.

Primers used for allele identification.

Allele	Strand	Sequence	Amplicon Size (bp)
Genotyping
Cdh5-Cre	Forward	5’-GCGGTCTGGCAGTAAAAACTATC -3’	100
	Reverse	5’-GTGAAACAGCATTGCTGTCACTT-3’
Dmp1-Cre	Forward	5’-CCCGCAGAACCTGAAGATG-3’	534
	Reverse	5’-GACCCGGCAAAACAGGTAG-3’
Hprt WT	Forward	5’-TTTCTATAGGACTGAAAGACTTGCTC-3’	200
	Reverse	5’-CACAGTAGCTCTTCAGTCTGATAAAA-3’
Hprt Cre	Forward	5’-GCGGTCTGGCAGTAAAAACTATC-3’	100
	Reverse	5’-GTGAAACAGCATTGCTGTCACTT-3’
Notch3	Forward	5’-GAGGCCCAAGGAATCGAGAC-3’	269
	Reverse	5’-ATGAGACGTTTTCTCCGAGTTCAG-3’
Notch3 COIN	Forward	5’-CCGGGCCGCGACTGAAACCCTAG-3’	158
	Reverse	5’-GGTGGGGCTACTGCGGGTTCCTG-3’
Sm22a-Cre	Forward	5’-GCGGTCTGGCAGTAAAAACTATC -3’	100
	Reverse	5’-GTGAAACAGCATTGCTGTCACTT -3’
LoxP Recombination
Notch3 INV	Forward	5’- CTGCTCCAGGACATGGAGAGG -3’	Non rec no band
	Reverse	5’- CACGTCGTAGGGGTACTGTAGG -3’	Rec = 495