Skip to main content
. 1998 Nov;66(11):5580–5586. doi: 10.1128/iai.66.11.5580-5586.1998

FIG. 2.

FIG. 2

Western immunoblot analysis of Tir-expressing clones using convalescent sera from HUS patients. Lysates of E. coli DH5α carrying pBluescript (lane 1), pJCP580 (lane 2), pJCP581 (lane 3), pJCP582 (lane 4), or pJCP583 (lane 5) or STEC 95NR1 (lane 6) were separated by SDS-PAGE, electroblotted, and probed with convalescent sera from HUS patients infected with O111:H− STEC (A and C), both O111:H− and O157:H− STEC (B), or a LEE-negative OR:H9 STEC (D). The mobility of protein mass markers is indicated to the left.