Table 1.
Primers used for detection of carbapenemase genes
| Gene | Primer DNA sequence | Amplicon size (bp) | Reference |
|---|---|---|---|
| bla NDM−1 |
F-5’GGTTTGGCGATCTGGTTTTC 3’ R-5’CGGAATGGCTCATCACGATC 3’ |
621 bp | [15] |
| bla KPC |
F-5’CGTCTAGTTCTGCTGTCTTG 3’ R-5’CTTGTCATCCTTGTTAGGCG3’ |
798 bp | [15] |
| bla OXA−48 |
F-5’GCGTGGTTAAGGATGAACA3’ R-5’CATCAAGTTCAACCCAACCG3’ |
438 bp | [15] |
| bla IMP |
F-5’GGAATAGAGTGGCTTAAYTCTC3’ R-5’GGTTTAAYAAAACAACCACC3’ |
232 bp | [16] |
| bla SPM |
F-5’AAAATCTGGGTACGCAAACG3’ R-5’ACATTATCCGCTGGAACAGG3’ |
271 bp | [16] |
| bla VIM |
F-5’GATGGTGTTTGGTCGCATA3’ R-5’CGAATGCGCAGCACCAG3’ |
390 bp | [16] |