FIG. 5.

Detection of the poxA gene in other bacterial species. Total DNA from S. typhimurium (5 μg), S. flexneri (10 μg), Y. enterocolitica (10 μg), K. pneumoniae (3 μg), P. aeruginosa (10 μg), M. tuberculosis (10 μg), E. rhusiopathiae (10 μg), B. burgdorferi (10 μg), and P. multocida (10 μg) was digested with the restriction enzyme ClaI. DNA fragments were separated on a 0.8% agarose gel and transferred to a GeneScreen Plus nylon membrane. The blot was hybridized to a fluorescein-labeled 1-kb BstBI-XhoI internal fragment of poxA as a probe. The hybridization was carried out at 50°C with stringent washes in 1× SSC–0.1% SDS. The limited amount of genomic DNA available from K. pneumoniae could account for the weak signal. Numbers at left show molecular mass in kilodaltons.