Figure 5.

CD8⁺ T cells transplantation aggravated apoptosis of ECs. (a) Representative immunostaining images of CD31 (green) with TUNEL (red) in the hemorrhagic perifocal area at day 3 of ICH in Oil ICH and Tam ICH groups. Scale bar = 50 μm. Arrowheads indicate the colocalization of CD31 with TUNEL. Number of apoptotic ECs in the hemorrhagic perifocal area at day 3 of ICH in Oil ICH and Tam ICH groups. n = 4 mice per group (all male). Two-sided, Welch’s t test. (b) Representative immunostaining images of CD31 (green) with TUNEL (red) in the hemorrhagic perifocal area at day 3 of ICH in Tam Sham + CD8⁺ T, Tam ICH, and Tam ICH + CD8⁺ T groups. Scale bar = 50 μm. Arrowheads indicate the colocalization of CD31 with TUNEL. (c) Number of apoptotic ECs in the hemorrhagic perifocal area at day 3 of ICH in Tam Sham + CD8⁺ T, Tam ICH, and Tam ICH + CD8⁺ T groups. n = 4 mice per group (2 female, 2 male). One-way ANOVA with Tukey’s multiple comparisons test. (d) Representative perfused whole brains and 2 mm brain sections after EB injection in Tam Sham, Tam Sham + CD8⁺ T, Tam ICH and Tam ICH + CD8⁺ T groups. Quantification of extravasated EB from brains after EB injection in Tam Sham, Tam Sham + CD8⁺ T, Tam ICH, and Tam ICH + CD8⁺ T groups. n = 4 mice in Tam Sham and Tam Sham + CD8⁺ T groups (3 female, 1 male), n = 5 mice in Tam ICH group (3 female, 2 male), n =5 mice in Tam ICH + CD8⁺ T group (2 female, 3 male). One-way ANOVA with Tukey’s multiple comparisons test and (e) Representative immunostaining images of IgG leakage in the hemorrhagic perifocal area in Tam Sham, Tam Sham + CD8⁺ T, Tam ICH, and Tam ICH + CD8⁺ T groups. Scale bar = 100 μm. F. Semi-quantification of IgG intensity in the hemorrhagic perifocal area in Tam Sham, Tam Sham + CD8⁺ T, Tam ICH, and Tam ICH + CD8⁺ T groups. n = 4 mice per group (2 female, 2 male). One-way ANOVA with Tukey’s multiple comparisons test. All data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.