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. 2023 Nov 16;44(3):367–383. doi: 10.1177/0271678X231214838

Figure 6.

Figure 6.

Inhibition of perforin reduced apoptosis of ECs. (a) Representative immunoblots of Perforin and Granzyme B protein expression levels in the hemorrhagic perifocal area in Oil Sham, Tam Sham, Oil ICH and Tam ICH groups. (b, c) Quantification of Perforin (b) and Granzyme B (c) protein expression levels in the hemorrhagic perifocal area in Oil Sham, Tam Sham, Oil ICH and Tam ICH groups. n = 3 mice per group (2 female, 1 male). β-actin was used as an internal control. One-way ANOVA with Tukey’s multiple comparisons test. (d) Statistics of bEnd.3 cells viability by CCK8 in Control, CD8+ T, CD8+ T + EGTA, and EGTA groups. n = 4 biologically independent bEnd.3 cell cultures. One-way ANOVA with Tukey’s multiple comparisons test. (e) Representative immunostaining images of CD31 (green) with TUNEL (red) in Control, CD8+ T, CD8+ T + EGTA, and EGTA groups. Scale bar = 50 μm. Arrowheads indicate the colocalization of CD31 with TUNEL and (f) Number of apoptotic bEnd.3 cells in Control, CD8+ T, CD8+ T + EGTA, and EGTA groups. n = 4 biologically independent bEnd.3 cell cultures. One-way ANOVA with Tukey’s multiple comparisons test. All data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.