Table 2.
Mean (± Standard Deviation) pocket volume (Å3) and solvent accessible surface area (Å2) in the active-site pocket for wild-type (WT) and its variants. T-statistics and p-values were derived from two-sample t-tests comparing each mutant with WT. p-values below 0.0001 are reported as ‘p < 0.0001’.
| Volume(Å3) | T-stat(Volume) | p-value(Volume) | SASA(Å2) | T-stat(SASA) | p-value(SASA) | |
|---|---|---|---|---|---|---|
| WT | 1071.81 ± 157.55 | 0.00 | 1.0000 | 154.99 ± 2.51 | 0.00 | 1.0000 |
| V211A | 1182.23 ± 200.20 | 75.08 | p < 0.0001 | 156.27 ± 3.44 | 52.03 | p < 0.0001 |
| V211G | 1181.68 ± 187.35 | 77.74 | p < 0.0001 | 154.82 ± 2.49 | −8.71 | p < 0.0001 |
| G214A | 1075.46 ± 259.80 | 2.08 | 0.0373 | 155.20 ± 2.73 | 9.46 | p < 0.0001 |
| G214R | 1115.24 ± 241.80 | 26.07 | p < 0.0001 | 156.65 ± 2.84 | 75.71 | p < 0.0001 |
| E219A | 1206.33 ± 221.88 | 85.62 | p < 0.0001 | 155.89 ± 2.77 | 41.51 | p < 0.0001 |
| E219G | 1211.74 ± 250.02 | 82.01 | p < 0.0001 | 155.99 ± 3.22 | 42.29 | p < 0.0001 |
| E219K | 1278.62 ± 293.01 | 107.67 | p < 0.0001 | 156.41 ± 2.69 | 66.74 | p < 0.0001 |
| Y221A | 1381.32 ± 302.32 | 157.25 | p < 0.0001 | 156.89 ± 2.95 | 84.61 | p < 0.0001 |
| Y221H | 1210.70 ± 182.42 | 99.80 | p < 0.0001 | 156.03 ± 2.47 | 51.27 | p < 0.0001 |