Table 2.
Mean (± Standard Deviation) pocket volume (Å3) and solvent accessible surface area (Å2) in the active-site pocket for wild-type (WT) and its variants. T-statistics and p-values were derived from two-sample t-tests comparing each mutant with WT. p-values below 0.0001 are reported as ‘p < 0.0001’.
| Volume(Å3) | T-stat(Volume) | p-value(Volume) | SASA(Å2) | T-stat(SASA) | p-value(SASA) | |
|---|---|---|---|---|---|---|
| WT | 1072 ± 158 | 0 | 1.0000 | 155 ± 3 | 0 | 1.0000 |
| V211A | 1182 ± 200 | 75 | p < 0.0001 | 156 ± 3 | 52 | p < 0.0001 |
| V211G | 1182 ± 187 | 78 | p < 0.0001 | 155 ± 2 | −9 | p < 0.0001 |
| G214A | 1075 ± 260 | 2 | 0.0373 | 155 ± 3 | 9 | p < 0.0001 |
| G214R | 1115 ± 242 | 26 | p < 0.0001 | 157 ± 3 | 76 | p < 0.0001 |
| E219A | 1206 ± 222 | 86 | p < 0.0001 | 156 ± 3 | 42 | p < 0.0001 |
| E219G | 1212 ± 250 | 82 | p < 0.0001 | 156 ± 3 | 42 | p < 0.0001 |
| E219K | 1279 ± 293 | 108 | p < 0.0001 | 156 ± 3 | 67 | p < 0.0001 |
| Y221A | 1381 ± 302 | 157 | p < 0.0001 | 157 ± 3 | 85 | p < 0.0001 |
| Y221H | 1211 ± 182 | 100 | p < 0.0001 | 156 ± 2 | 51 | p < 0.0001 |