Fig. 1:

Representative photomicrographs of baboon astrocyte, fibroblast, and hepatocyte cultures. (A) Astrocytes derived from prefrontal cortex and hippocampus show immunoreactivity for astrocyte markers; aquaporin 4, glial fibrillary acidic protein (GFAP), and excitatory amino acid transporter 2 (EAAT2). The absence of immunoreactive signals for myelin basic protein and post synaptic density protein 95 (PSD95) indicates that astrocyte cultures do not contain oligodendrocytes and neurons, respectively. (B) Expression of fibroblast marker, vimentin in fibroblasts from different heart regions such as the left ventricle, right ventricle, left atrium, and right atrium as well as skin epidermal fibroblasts express fibroblast marker, vimentin. (C) Phase contrast microscopy of hepatocyte cultures and immunoreactive signals of epithelial cell marker, cytokeratin 8. Scale 20 µm. Images acquired with a Zeiss LSM 880 confocal microscope using a 63x oil immersion lens. Donor animal: Male, 16.4 years old.