TABLE 1.
Primers used to amplify various regions of the eae genes from O127:H6 and O114:H2
| pMal-Int construct | Primer
|
|
|---|---|---|
| Forwarda | Reverseb | |
| Int80α | CTGAATTCGCCAGCATTACTGAGATTAAG | CTTCTAGATTAGACATCGCTAACACGGGCAC |
| Int40-(1)α | CTGAATTCGCCAGCATTACTGAGATTAAG | CTTCTAGATTACGTCGTAAAGGTCACTTCCTG |
| Int40-(2)α | CTGAATTCACGACCTTAGCTAAGTTAAGTAATCC | CTTCTAGATTAGACATCGCTAACACGGGCAC |
| Int40-(3)α | CTGAATTCTACACTGTTAAAGTGATGAAGGGG | CTTCTAGATTATGTTACTTTGGCATAGCCATTCG |
| Int80β | CTGAATTCGCCAGCATTACTGAGATTAAG | CTTCTAGATTAACTCACTTTTGCACTAACAATG |
| Int130β | CTGAATTCGCCAGCATTACTGAGATTAAG | CTTCTAGATTAATTGCCCCCTGTTGTTGCCTGTAC |
| Int150β | CTGAATTCAATGCAAAATACACATGGAAATCCAGTAAAAC | CTTCTAGATTATTTTACACAARYKGCAWAAGC |
| Int100β | CTGAATTCGGTACAGAAGTTAAGGCTACTACC | CTTCTAGATTAACTACCCGGTGCATTTAATGTGT |
| Int120β | CTGAATTCACAATTACTGTAGTATCTGGTGATC | CTTCTAGATTATTTTACACAARYKGCAWAAGC |
| Int70β | CTGAATTCTCAAAAGAACTATTGGCCATTATC | CTTCTAGATTATTTTACACAARYKGCAWAAGC |
| Int40-(1)β | CTGAATTCGCCAGCATTACTGAGATTAAG | CTTCTAGATTACTTAGAAAAGGTCACTTTCTGATG |
| Int40-(2)β | CTGAATTCAAGGATTTTGGGACCCTGAATAAG | CTTCTAGATTAACTCACTTTTGCACTAACAATG |
| Int40-(3)β | CTGAATTCTATACTGTCAGAGTGATGAAGG | CTTCTAGATTATACAGTAGCATAACCATTCTCATCG |
a
Underlined sequences represent the EcoRI site.
b
Underlined sequences represent the XbaI site.