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Dose-dependent binding of STb to sulfatide. Sulfatide was immobilized on a PVDF membrane at various concentrations ranging from 0.001 to 2 μg in 1 μl of methanol, and the binding of STb (0.1 μg/ml) was measured after 10 min as described in Materials and Methods. Values represent the averages of duplicate experiments (three spots per experiment) analyzed by densitometry. The standard deviations from the mean are shown.
