FIG. 3.
Effects of various chemicals on STb binding to sulfatide. Sulfatide (1 μg) was immobilized on a PVDF membrane. Incubation of STb (0.1 μg/ml) was performed for 10 min with either 0.05 or 0.1 M TMU or 0.05 or 1 mg of dextran (DX; Mr, 9500) or dextran sulfate (DS; Mr, 10000) per ml before addition to immobilized sulfatide; binding of STb toxin was measured after 10 min as described in Materials and Methods. Values represent the averages of duplicate experiments (three spots per experiment) analyzed by densitometry. The standard deviations from the mean are shown.