FIG. 7.

Binding of STb to the sulfatide isolated from pig jejunal epithelial cells. Following extraction from preparative TLC developed in chloroform–methanol–0.02% CaCl₂ in water (55:45:10 by volume), the eluted pig jejunal sulfatide was immobilized on a PVDF membrane, and the binding of STb was measured after 10 min as described in Materials and Methods. Binding was calculated by subtracting the mean DU obtained for the silica gel control from the mean DU obtained for the eluted sulfatide. Values represent the averages of triplicate experiments (three spots per experiment) analyzed by densitometry. The standard deviations from the mean are shown.