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Sodium dodecyl sulfate–Tricine–15% polyacrylamide gel showing in vitro activity of VvpD on P. aeruginosa prepilin substrate. Membranes were prepared from V. vulnificus strains carrying the plasmids indicated. Lanes: A, prepilin substrate only; B, C7184(pMMB67HE.cam); C, C7184D12Ω(pMMB67HE.cam); D, C7184D12Ω(pRPD1). Positions of molecular weight markers are shown on the left in kilodaltons.
