TABLE 2.
Secretion of cytolysin, protease, and chitinase in V. vulnificus
| Strain | Hemolytic indexa
|
Extracellular secretionb
|
|||
|---|---|---|---|---|---|
| Supernatant | Periplasm | Cytoplasm | Protease | Chitinase | |
| C7184(pMMB67HE.cam) | 2,048 | 0 | 0 | + | + |
| C7184D12Ω(pMMB67HE.cam) | 8 | 272 | 4 | − | − |
| C7184D12Ω(pRPD1) | 2,048 | 16 | 4 | + | + |
| C7184-402K | 0 | 0 | 0 | ND | ND |
a
Reciprocal of the highest dilution of culture supernatant or cell fraction at which hemolysis was observed (average of two assays).
b
Detected by a zone of clearing around the bacterial colonies (see Materials and Methods). ND, not done.