TABLE 4.
Cytotoxicity, adherence, and virulence
| V. vulnificus strain | CHO cell cytotoxicitya | HEp-2 cell adherence (%)b | Mouse LD50 |
|---|---|---|---|
| C7184(pMMB67HE.cam) | 64 | 9.2 ± 2.1 | 9.4 × 103 |
| C7184-D12Ω(pMMB67EH.cam) | <2 | 0.3 ± 0.1c | 1.1 × 106 |
| C7184-D12Ω(pRPD1) | 64 | 4.5 ± 2.0c | 2.1 × 105 |
| C7184-402K | 64 | 9.0 ± 3.6 | NDd |
a
Reciprocal of the highest dilution of filtered bacterial supernatants to cause typical CHO cell cytotoxicity (cell lysis, rounding, and detachment of monolayer) after 6 h.
b
Presented as (number of CFU recovered/number of input CFU) × 100 ± standard deviation.
c
Significantly different when compared by Tukey-Kramer analysis at P ≤0.5.
d
ND, not done.