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. 2023 Sep 9;79(3):589–605. doi: 10.1097/HEP.0000000000000590

FIGURE 6.

FIGURE 6

Aberrant neutrophils infiltration and NET formation promoted by aged intrahepatic macrophages to exacerbate liver damage in the HIRI model. (A) Schematic displaying the experimental design. Aged mice were pretreated with CLLs to deplete macrophages for 48 h, and the HIRI model was subsequently established, followed by a single intrasplenic injection of young intrahepatic macrophages (Y→A chimera, n = 4). (B) Histology (H&E staining, upper), TUNEL staining (middle), and IHC staining for Ly6G (lower) in liver tissues in each group. Scale bar: 100 μm. (C) The severity of liver injury in each group was scored according to Suzike’s injury criteria (upper panel). Statistical analyses of the percent of TUNEL-positive cells (middle panel) and neutrophils (lower panel) in liver tissues in each group. (D) The levels of sALT, sAST, and sLDH in each group. (E) Statistical analyses of NET formation in liver tissues in each group. (F) Histology (H&E staining, upper), TUNEL staining (middle), and IHC staining for Ly6G (lower) in liver tissues in each group. Scale bar: 100 μm. (G) Statistical analyses of Suzike’s injury criteria (upper), the percentage of TUNEL-positive cells (middle), and the number of neutrophils (lower) in each field (n = 4). (H) The levels of sALT, sAST, and sLDH in mice in the sham, anti-IgG, and anti-CXCL2 groups. (I) Statistical analyses of NET formation in liver tissues in each group. Data are presented as the means ± SD. Abbreviations: CLL, clodronate liposomes; HIRI, hepatic ischemia-reperfusion injury; NETs, neutrophil extracellular traps; sALT, serum alanine aminotransferase; sAST, serum aspartate aminotransferase; sLDH, serum lactate dehydrogenase.