Figure 2.
Livers of IR-ASO–treated mice contain less glycogen than livers of animals treated with control-ASO. H&E staining of frozen sections of livers obtained from fasted mice treated with 100 mg/kg body weight of control-ASO (A) or IR-ASO (B) failed to reveal any major structural abnormalities. However, PAS staining suggested a decrease in glycogen content in livers of IR-ASO–treated mice (D) compared with livers of control-ASO–treated animals (C). This decrease was confirmed by chemical analyses of multiple liver samples (E) from the 2 experimental groups (#P < 0.001; IR-ASO vs. control-ASO).