FIGURE 1.

tMNVs restore miR-126-3p expression in liver tumor cells. (A, B) HepG2 and SNU449 cells were incubated with 2×10¹¹ MNVs loaded with a nontarget control miRNA (control MNVs) or pre-miR-126-3p (tMNVs), and the relative expression of (A) miR-126 and (B) ADAM9 was assessed after 48 hours. (C, D). Media was collected from HepG2 or SNU449 cells treated with control or tMNVs for 48 h, or from untreated cells. (C) The levels of secreted soluble MICA were quantified by ELISA, and (D) mMICA expression was evaluated by flow cytometry. The values are expressed as the mean±SD from 3 replicates. *p < 0.05, **p <0.01, ***p<0.001. Abbreviations: MICA, major histocompatibility complex I-related chain A; mMICA, membrane-bound MICA; tMNVs, therapeutic MNVs.