Figure 6.

Endothelial cells have a marked increase in CXCR2 expression during the pathogenesis of murine BOS. (A) Real-time quantitative PCR determination of CXCR2 mRNA expression presented as fold increase in chemokine expression in allografts compared with syngeneic controls at days 3, 7, 14, 21, and 28. *P < 0.05. (B) FACS analysis of gated MECA-32 cells from tracheal allografts for cell surface protein expression of CXCR2 at days 7 and 21. (C) Representative photomicrographs of immunolocalization of CXCR2 in a murine fibro-obliteration lesion at day 21 compared with control Abs. Magnification, ×400.