FIG. 8.

Binding inhibition assay. Inhibition of B. forsythus binding to surface-coated matrix components by rBsp70. Microtiter plate wells coated with matrix components were incubated with B. forsythus cells in the presence of increasing concentrations of the rBsp70 protein. Bound cells were detected by an anti-B. forsythus antibody, followed by a second antibody coupled to peroxidase, and color development with TMB. Percent inhibition of B. forsythus cell binding to each matrix component was plotted against the rBsp70 concentration. The results shown are means of duplicate samples that are representative of several experiments.