FIG. 9.

Immunoblot analysis of the B. forsythus membrane fraction. SDS-PAGE-separated proteins were transferred onto nitrocellulose membranes and probed with a rabbit anti-rBsp70 antibody and goat anti-rabbit IgG coupled to HRP. The enzyme reaction was developed with the membrane substrate TMB. Lanes: 1, rBsp70; 2, E. coli cell extract containing pBluescrioptSK⁻ (used as negative control); 3, E. coli extract harboring pBBf4 (positive clone); 4, B. forsythus membrane fraction; 5, B. forsythus whole-cell extract; 6, culture medium of actively growing B. forsythus cells. The values on the left are molecular sizes in kilodaltons.