Figure 2.

Time-lapse microscopy analysis of CLB2 overexpressors. Wild-type CLB2/CLB2, 1× GAL-CLB2/CLB2, and 2× GAL-CLB2/CLB2 strains, all containing a copy of TUB1-GFP and CDC10-GFP, were grown to log phase in Sc-raffinose medium, preinduced with galactose for 1 h, and then plated on Sc-galactose slabs. Time-lapse microscopy was carried out for 6 h with GFP detection every 3 min. (A) Still images from the 3-h time point. (B) Cartoon of the cell cycle with septin ring (collar between cell and bud, labeled by Cdc10-GFP) and spindle (dot and line, labeled by Tub1-GFP), with the relevant intervals indicated. (C) Approximate quantitation of cell cycle intervals for all scoreable cells in the movies, mean and SD. See Materials and Methods for more information on scoring.