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Cdc48 is required for ER transitions. Time-lapse confocal microscopy of RNAi treated SP12::expressing embryos. The knockdown of NSF-1 and 2 Cdc48 homologues does not interfere with ER cycling. RNAi of NSF-1 does lead to a more granular ER over time. Knockdown by RNAi of Cdc48 (K04G2.3) causes the ER to collapse. The first row depicts a time-lapse 24 h and the second row 48 h postinjection. Bars, 10 μm.
