Figure 1.
Deduced sequence of Dictyostelium WASP. (A) Sequence comparison of Dictyostelium WASP with WASPs from other species. Deduced amino acid sequence of WASP from the 1200-base pair DNA clone is aligned with human (Hs), mouse (Mm), Caenorhabditis elegans (Ce), and Drosophila (Dm) WASP. (B) Schematic diagram of WASP domain structure. WH1, WASP homology I domain; B, Basic domain; GBD, Cdc42/Rac binding domain; V, Verprolin homology domain; C, central domain; A, acidic domain. (C) Northern blot showing the developmental time course of WASP expression. Total RNA of 16 μg/sample was resolved on a 1.0% denaturing agarose gel, blotted, and probed as described previously (Datta and Firtel, 1987). The 0-h time point is for vegetative cells. (D) Northern blot showing reduction of WASP transcript in WASPhypo cells and the lack of WASP transcript in WASPTK cells.