Table 1.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pUA4 | sec22-R157Q in pRS313 (CEN6-HIS3) | H. D. Schmitt |
| pUA8 | SEC22 in pRS313 (CEN6-HIS3) | H. D. Schmitt |
| pSPT18-SLY12 | 1.8-kb BET1 HindIII/NcoI in pSPT18 | Dascher et al. (1991) |
| pCG36 | 1.8-kb BET1 Hind III/SacI in pRS316 (CEN6-URA3) | This study |
| pCG27 | 1.8-kb containing bet1-S86R HindIII/SacI in pRS316 (CEN6-URA3) | This study |
| pCG39 | 1.8-kb containing bet1-S86Q in pRS316 (CEN6-URA3) | This study |
| pCG64 | 1.8- + 1.5-kb bet1-S86R XbaI/SacI, sec22-R157Q in pRS313 (CEN6-HIS3) | This study |
| pUA43 | SED5 in pRS325 (2 μ-LEU2) | H. D. Schmitt |
| pCG34 | 3-kb SED5 in pRS316 (CEN6-URA3) | This study |
| pCG32 | 3-kb sed5-Q283R in pRS316 (CEN6-URA3) | This study |
| pCG51 | 3-kb sed5-Q283R in pRS315 (CEN6-LEU2) | This study |
| pCG163 | 3-kb sed5-Q283R in pRS317 (CEN6-LYS2) | This study |
| pCG62 | 3- + 1.5-kb sed5-Q283R SalI in XhoI, sec22-R157Q in pRS313 (CEN6-HIS3) | This study |
| pUA37 | BOS1 in pRS315 (CEN6-LEU2) | Andag et al. (2001) |
| pCG69 | 1.8-kb bos1-Q186R in pRS315 (CEN6-LEU2) | This study |
| pCG161 | 1.8-kb bos1-Q186R in pRS316 (CEN6-URA3) | This study |
| pTN75 | UFE1 in pRS316 (CEN6-URA3) | H. D. Schmitt |
| pCG5 | 1.8-kb ufe1-Q289R in pRS316 (CEN6-URA3) | This study |
| pUA65 | YKT6 in pRS315 (CEN6-LEU2) | H. D. Schmitt |
| pCG165 | YKT6 BamHI/XhoI in pRS316 (CEN6-URA3) | This study |
| pBK87 | 1.1-kb ykt6-R165Q in pRS315 (CEN6-LEU2) | Dilcher et al. (2001) |
| pCG60 | ykt6-R165Q BamHI/EcoRI in pRS316 (CEN6-URA3) | This study |
| pCG170 | 1.8-kb bet1-S86Q XhoI/KpnI in pCG60 (CEN6-URA3) | This study |
| pCG172 | 1.8-kb bet1-S86Q XhoI/KpnI in pCG165 (CEN6-URA3) | This study |